S-STEM Scholar Rebecca Matadamas's Blog

on 1/26 I finally did a gram stain of gobi that is clearly gobi. It's been a while since I got a good look of gobi without burning it during the heat fix. Really pretty purple grapes. Soon, we'll be growing biofilm from this.

10/16 was another day of filling a well plate with acetic acid. 10/19, my lab partner and I filled three new wells plates with our 3 salts and our D. Gobi, just like our original two plates were, with an added combination this time (AB+AC+BC). Each plate had 2 salts in that combination.

For our first plate that went through the CV Assay, it had dried overnight and was ready to have acetic acid solubilize it. Baylee used 30% acetic acid and filled each well plate with 130 microliters of this acid. We waited 15 minutes, then she transferred 125 microliters of each sample from the original well plate to a new plate. Then we had to wait for a few days for that to dry.

On 10/8, we came in to add our D. Gobi to some TGY to prepare for filling our well plates. I unfortunately had to abandon my partner for class, but she did wonderfully, as usual. On 10/12, we began our CV Assay for Plate 1. There was a lot of CV stain left on the well plate after all the washing, which does not show very well in picture, but was exciting for us. The picture below is after the CV had sit to dry overnight. On 10/13, we did our CV Assay for Plate 2. The process was exactly the same for Plate 2 as it was for Plate 1.

During this lab, I was streaking some of our TGY plates with our D. Gobi. My lab partner, Baylee, did an O.D reading of our O/N sample, and it came out too low. We had to have it spun down so the cells would be more concentrated.

(many things that happened this weekend had to be redone because I think it rained) We inoculated 3mL TGY into a falcon tube and then inoculated some of our Gobi sample into the TGY. I did a gram stain and realized I tend to burn my samples so this is a slide of Gobi, just really crispy.