Lab 4/2 and 4/7

On 4/2 I performed my first gram stain. It was nice to actually do something that is talked about so casually in the lab. I gram stained 3 different samples, two aquaticus and one fluorescens. This picture is of the fluorescence sample, showing it to be gram negative.
On 4/6, we did an inoculation of transformed e. coli to prep for our 4/7 plasmid prep/isolation.
On 4/7 we did out plasmid prep, and one of our samples from the day before did not grow, so we were pulling from only one sample. By the end, we saw that our samples were pretty dirty, having a 260/280 ration of 2.21 and 2.08, but it was expected, being our first prep.


Comments

  1. Claudia MillanesApril 10, 2018 at 6:01 PM

    Your gram stain looks amazing! Great job on it, and it was your first. I believe to be amazing how we can figure out what kind of bacteria it is with the colors it retrains.

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  2. Ricardo M.April 12, 2018 at 9:23 PM

    That's a really cool gram stain. I wonder what aquaticus and fluorescence are...definitely, those are some gram negative bacteria that can be identified because of the red absorption of the red dye. That's really cool that you guys are using Ecoli, that a really handy bacteria to use for inserting plasmids and introducing those genes to other bacteria.

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