(many things that happened this weekend had to be redone because I think it rained) We inoculated 3mL TGY into a falcon tube and then inoculated some of our Gobi sample into the TGY.
I did a gram stain and realized I tend to burn my samples so this is a slide of Gobi, just really crispy.
3/24/18 was my first day in the STEM lab and in a biology lab, so sorry if this post is a bit confusing or does not make sense because my concept of biology is fairly weak. In this picture, it shows a putative lactonase sample. On line 2, there was no DNA; it was clean. On lines 3 and 4, it shows the bands seen at approximately 800 bpm, and in line 5 there was no band present. In this lab, I learned a lot about the micro-pipets and which ones to use during specific steps of this process. I am still very new to all of this, so hopefully my future posts will be more understandable.
10/16 was another day of filling a well plate with acetic acid. 10/19, my lab partner and I filled three new wells plates with our 3 salts and our D. Gobi, just like our original two plates were, with an added combination this time (AB+AC+BC). Each plate had 2 salts in that combination.
During this lab weekend, my lab partner, Baylee, and I began planning out our biofilm experiment with Chad, one of the peer mentors in this STEM lab. After researching much about biofilm and effects that can vary the growth or reduction of biofilm, my lab partner and I decided testing the effect of salt concentration on biofilm would gather information that may not already be out there. For this experiment, we will be using D. Gobi, and testing salts MgCl ₂, MnCl ₂ , and NaCl.
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