(many things that happened this weekend had to be redone because I think it rained) We inoculated 3mL TGY into a falcon tube and then inoculated some of our Gobi sample into the TGY.
I did a gram stain and realized I tend to burn my samples so this is a slide of Gobi, just really crispy.
10/16 was another day of filling a well plate with acetic acid. 10/19, my lab partner and I filled three new wells plates with our 3 salts and our D. Gobi, just like our original two plates were, with an added combination this time (AB+AC+BC). Each plate had 2 salts in that combination.
3/24/18 was my first day in the STEM lab and in a biology lab, so sorry if this post is a bit confusing or does not make sense because my concept of biology is fairly weak. In this picture, it shows a putative lactonase sample. On line 2, there was no DNA; it was clean. On lines 3 and 4, it shows the bands seen at approximately 800 bpm, and in line 5 there was no band present. In this lab, I learned a lot about the micro-pipets and which ones to use during specific steps of this process. I am still very new to all of this, so hopefully my future posts will be more understandable.
During this lab, we were subculturing. We put aquaticus German in TGY, Caenie in R2A, Deserti in TSB/10, Hopi in TGY, Grandis in PYEA, Pima in R2A, D. Rad in TGY, Sonoraensis in R2A, Indicus in TSYE, and Geothermalis in NA. It went well and now we wait for them to grow.
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